Journal:

Article Title: Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo

doi: 10.1101/gad.486108

Figure Lengend Snippet: Elevation of Cdx2 expression promotes symmetric divisions. Cdx2 was overexpressed in one late two-cell/early four-cell blastomere, and embryos were followed by time-lapse microscopy to the blastocyst stage (two sample embryos shown in A–G and H–N). In controls, DsRed mRNA only was injected (O–V). Lineages were generated with SIMI Biocell software, and the centers of the nuclei from each clone are marked either red (injected clone) or blue (wild-type clone). From 16-cell onward, nuclei of inside cells are marked yellow (injected clone) or light blue (wild-type clone); see W for coding. Cdx2 expression leads to significantly more symmetric than asymmetric divisions (indicated by the relative numbers of yellow versus light blue cells). Merged 3D representations and DIC images from three different embryos are shown in A–G, H–N, and O–V. Times of images are in minutes. Schematic representation of lineage trees was generated with SIMI Biocell software (X; embryo A–G, Y; embryo H–N). (Z) Proportions of symmetric or asymmetric divisions taken by clones expressing injected Cdx2 mRNA or DsRed mRNA relative to the clone derived from the noninjected cell (see also Supplemental Table 2).

Article Snippet: Schematic representation of lineage trees was generated with SIMI Biocell software (X; embryo A – G , Y; embryo H – N ). ( Z ) Proportions of symmetric or asymmetric divisions taken by clones expressing injected Cdx2 mRNA or DsRed mRNA relative to the clone derived from the noninjected cell (see also Supplemental Table 2).

Techniques: Expressing, Time-lapse Microscopy, Injection, Generated, Software, Clone Assay, Derivative Assay

Journal:

Article Title: Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo

doi: 10.1101/gad.486108

Figure Lengend Snippet: Down-regulation of Cdx2 expression leads to a reduced contribution to the trophectoderm. Cdx2 was down-regulated in half of the embryo by dsRNA injection to one blastomere. (A–H) Sections through fixed and immunocytochemically stained embryos at the morula (A–D) and blastocyst (E–H) stage show specific knockdown of Cdx2 protein in the injected clone; the white dashed line indicates injected cell progeny. (I) Schematic of lineage trees generated with SIMI Biocell software. (J–O) Embryos were followed by time-lapse microscopy to the blastocyst stage. Merged 3D representations and DIC images are shown; times of images are in minutes. Color-coding as in Figure 2. (P) Proportions of symmetric/asymmetric divisions taken by the Cdx2 RNAi-injected clone relative to the wild-type clone at fourth and fifth cleavage rounds (see also Supplemental Table 3A).

Article Snippet: Schematic representation of lineage trees was generated with SIMI Biocell software (X; embryo A – G , Y; embryo H – N ). ( Z ) Proportions of symmetric or asymmetric divisions taken by clones expressing injected Cdx2 mRNA or DsRed mRNA relative to the clone derived from the noninjected cell (see also Supplemental Table 2).

Techniques: Expressing, Injection, Staining, Knockdown, Generated, Software, Time-lapse Microscopy

Journal:

Article Title: Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo

doi: 10.1101/gad.486108

Figure Lengend Snippet: Elevation of Cdx2 expression promotes symmetric divisions. Cdx2 was overexpressed in one late two-cell/early four-cell blastomere, and embryos were followed by time-lapse microscopy to the blastocyst stage (two sample embryos shown in A–G and H–N). In controls, DsRed mRNA only was injected (O–V). Lineages were generated with SIMI Biocell software, and the centers of the nuclei from each clone are marked either red (injected clone) or blue (wild-type clone). From 16-cell onward, nuclei of inside cells are marked yellow (injected clone) or light blue (wild-type clone); see W for coding. Cdx2 expression leads to significantly more symmetric than asymmetric divisions (indicated by the relative numbers of yellow versus light blue cells). Merged 3D representations and DIC images from three different embryos are shown in A–G, H–N, and O–V. Times of images are in minutes. Schematic representation of lineage trees was generated with SIMI Biocell software (X; embryo A–G, Y; embryo H–N). (Z) Proportions of symmetric or asymmetric divisions taken by clones expressing injected Cdx2 mRNA or DsRed mRNA relative to the clone derived from the noninjected cell (see also Supplemental Table 2).

Article Snippet: Time-lapse imaging and analysis Lineages were followed by time-lapse microscopy and analyzed with SIMI Biocell software (for details, see Bischoff et al. 2008 ).

Techniques: Expressing, Time-lapse Microscopy, Injection, Generated, Software, Clone Assay, Derivative Assay

Journal:

Article Title: Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo

doi: 10.1101/gad.486108

Figure Lengend Snippet: Down-regulation of Cdx2 expression leads to a reduced contribution to the trophectoderm. Cdx2 was down-regulated in half of the embryo by dsRNA injection to one blastomere. (A–H) Sections through fixed and immunocytochemically stained embryos at the morula (A–D) and blastocyst (E–H) stage show specific knockdown of Cdx2 protein in the injected clone; the white dashed line indicates injected cell progeny. (I) Schematic of lineage trees generated with SIMI Biocell software. (J–O) Embryos were followed by time-lapse microscopy to the blastocyst stage. Merged 3D representations and DIC images are shown; times of images are in minutes. Color-coding as in Figure 2. (P) Proportions of symmetric/asymmetric divisions taken by the Cdx2 RNAi-injected clone relative to the wild-type clone at fourth and fifth cleavage rounds (see also Supplemental Table 3A).

Article Snippet: Time-lapse imaging and analysis Lineages were followed by time-lapse microscopy and analyzed with SIMI Biocell software (for details, see Bischoff et al. 2008 ).

Techniques: Expressing, Injection, Staining, Knockdown, Generated, Software, Time-lapse Microscopy